Oxidative Phosphorylation in Extracts of Rhodospirillum rubrum

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Oxidative phosphorylation in extracts of Rhodospirillum rubrum.

The purple photosynthetic bacterium, Rhodospirillum rubrum, grows anaerobically only when illuminated with light of appropriate wave lengths. Extracts prepared from cells grown in this manner catalyze an anaerobic light-dependent phosphorylation of adenosine diphosphate to adenosine triphosphate (1). However, R. rubrum also can be grown heterotrophically in darkness if oxygen is provided (2). U...

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Respiration and light-induced phosphorylation in extracts of Rhodospirillum rubrum.

As in many other kinds of bacteria, the addition of oxygen to anaerobic photosynthetic bacteria in the dark results in the oxidation of the cytochrome pigments of the respiratory chain (1, 2). Illumination of the anaerobic bacteria results in the oxidation of the respiratory chain cytochrome system as well as at least one additional cytochrome pigment (2). This type of observation, and work on ...

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Photophosphorylation in extracts of Rhodospirillum rubrum.

Preparations from a variety of photosynthetic organisms carry out a light-dependent phosphorylation of adenosine diphosphate. These systems appear to be similar in one respect: photophosphorylation is stimulated by substances that may behave as reducing agents, or as electron carriers. In the present study, the photophosphorylation system in extracts of Rhodospirillum rubrum is found to be acti...

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Correlation of light-induced absorbance changes with photo-phosphorylation in Rhodospirillum rubrum extracts.

The relationship between light-induced absorbance changes and photophosphorylation has been studied in extracts of the photosynthetic bacterium Rhodosfiirillum rubrum purified by partition in dextran-methyl cellulose mixtures. In these preparations infrared light induces absorbance changes in the visible region of the spectrum which are modified by the addition of reductants required for photop...

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Photosynthesis in Rhodospirillum rubrum

Ribulose 1,5-diphosphate carboxylase has been isolated from autotrophically cultured Rhoclospirillum rubrum. The molecular weight is 120,000. The K, for ribulose 1,5diphosphate is 83 mM, and for CO2 is 59 mM. The enzyme is inhibited by three important metabolites: citrate, an intermediate of the tricarboxylic acid cycle; inorganic phosphate; and 3-phosphoglyceric acid, the product of the reacti...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1962

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)60254-6